How do you preserve a cytology specimen?
The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological detail was well preserved in a variety of samples including fine needle aspirates, urine, and bronchial brushings.
Do cytology samples need to be refrigerated?
If the sample cannot be submitted directly to Cytopathology, the sample must be refrigerated until it can be delivered, along with the appropriate requisition form. 3. Fine needle aspiration may also be used for breast masses (please see below).
How do you store a cytology slide?
Place one slide in a cardboard slide folder to air dry. 7.) Place the remaining slide in a slide holder containing 95% alcohol. Note: It does not matter which slide is air dried and which slide is alcohol fixed.
Where do you store a specimen?
To increase the sensitivity of diagnostic testing all specimens should be stored frozen (optimally at -70oC) and shipped on dry ice with the exception of fixed tissues and specimens collected in a glass tube (whole blood, whole blood EDTA, whole blood Heparin, serum separating tubes, CPT tubes, etc).
How do you perform a cytology?
Another cytology technique is to gently scrape or brush some cells from the organ or tissue being tested. The best-known cytology test that samples cells this way is the Pap test. A small spatula and/or brush is used to remove cells from the cervix (the lower part of the uterus or womb) for a Pap test.
How many weeks are cytology samples stored?
Samples should be immediately prepared from the specimen. Record the date of preparation on the specimen container and refrigerate any remaining specimen. Specimen can be stored for one week before disposal.
What must be done to samples received in cytology in alcohol?
Liquid-based cytology involves pre-fixing the sample in an alcohol-based fixative before making the preparation in a machine which filters the fluid from the cells then pushes the cell deposit in a thin layer onto a prescribed area of a slide.
What cytology is used for?
Cytology is the exam of a single cell type, as often found in fluid specimens. It’s mainly used to diagnose or screen for cancer. It’s also used to screen for fetal abnormalities, for pap smears, to diagnose infectious organisms, and in other screening and diagnostic areas.
How long can a Specimen sit in formalin?
If you work with antibodies for clinical use, they work without changes in the IHC-protocol on formaldehyde fixed tissue up to 6 weeks. Longer fixation may need harsher retrieval.
How do you fix cytology smear?
2 The commonly used methods are air-dried and wet-fixed smears. Air- dried smears have many advantages over wet-fixed smears during routine cytology. They may be post- fixed after rehydration in saline with a variety of fixatives, such as ethanol/acetic acid, 95% ethanol or alcoholic formalin.
What are the stains used in cytology?
The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of OG6 and EA50 give the subtle range of green, blue and pink hues to the cell cytoplasm.
What is the best time to collect cytological materials?
Cytology specimens received in the laboratory after 2:30 p.m. on weekdays and on weekends or holidays will be evaluated by laboratory staff to see that they are properly preserved to prevent cellular degeneration.